New Glacios 2 Cryo-TEM with Low-Energy-Spread Cold FEG

The Thermo Scientific™ E-CFEG, a low-energy-spread cold field emission source launched as a value added component for the company’s highest-powered 300kV cryo-transmission electron microscope (cryo-TEM), is now also an option for the 200kV Thermo Scientific Glacios™ 2 Cryo-TEM.

This expanded offering is designed to help propel accessibility to cryo-electron microscopy (cryo-EM) techniques that boost resolution while maintaining ease-of-use and productivity capabilities. With a narrow energy spread to support high contrast images, the addition of the E-CFEG to the Glacios 2 Cryo-TEM enables near-atomic structural determination of complex proteins and macromolecules, and therefore, accelerated cryo-EM research and drug discovery processes using a lower accelerating voltage.

New data from Thermo Fisher Scientific using this combination demonstrates its ability to generate a 1.5 Å resolution structure of Apoferritin. This is currently the highest resolution structure generated from a commercially available cryo-TEM operating at 200kV.

In a package that reduces workflow complexity for users of various experience levels for single particle analysis, cryo-electron tomography and Micro-ED workflows, the platform add-on also includes AI-enabled algorithms for data collection and automated microscope alignments for increased ease of use to enable users to produce consistently excellent data. 

Key Applications

For structural biologists in biopharmaceutical and academic research who need high quality structural data at high throughput with more automation for improved efficiency and ease of use.    

Features/Benefits

• Powerful imaging potential to enable structure determination with improved resolution at <2A.
• Rapid data acquisition and data analysis with automated features to steer data collection, reduce idle time and enable users of a wide range of expertise levels to obtain high quality cryo-EM data.
• A smooth imaging experience with reduced effort due to a streamlined interface with built-in self-correction function to assist with microscope alignment and traffic light functionality for microscope readiness.