Lab Equipment > Biotech / Life Science > RNAi Technology > Nucleofector II (Amaxa Lonza) Electroporator - EXCELLENT CONDITION
For Sale: Nucleofector II (Amaxa Lonza) Electroporator - EXCELLENT CONDITION
Posted: Wednesday, May 1, 2013 Location: Austin, Texas, USA
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Ad Details - #492575
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Nucleofector II (Amaxa Biosystems / Lonza) electroporation device. Instrument is located in active cell biology lab and is in perfect working condition. 100% functional. Tested with over 8 different cell lines and works great. Routinely used fro nucleofection of K562, Jurkat, and primary T-cells.
Serial Number: 10700291
TESTED and VALIDATED system located in active biotechnology lab. Complete system! Just plug in and start working! We will include Amaxa cuvettes and buffer kit!
Instrument manual can be found on Amaxa (now Lonza) website or at this link: http://icob.sinica.edu.tw/pubweb/Core%20Facilities/Data/R401-core/Nucleofector_Manual_II_Apr06.pdf
Information about Nucleofector II system:
Transfection is an essential tool for numerous applications, including studies of gene expression. However, transfection of cells with common methods such as calcium phosphate, DEAE-dextran, liposome-mediated transfer, electroporation or viral vectors is limited because of low transfection efficiency and the extended time required for analysis of gene expression. Amaxa Biosystems has a new electroporation-based transfection method that enables the DNA to directly enter the nucleus while cells are stabilized in an electric field by treatment with a specific solution. The method is called “Nucleofection” and the electoporator system is called Nucleofector II. The system has built-in optimized programs for most common cell types.
The transfection procedure is simple and straightforward with these basic steps: (1) Preparing cells to be transfected with the nucleofection solution appropriate for the cell type, (2) Adding the nucleic acid to the prepared cells and transferring the mixture to a cuvette (provided in the kit), (3) Placing the cuvette in the system, electroporating cells with the optimized program for the cell type, (4) Harvesting cells from the cuvette and dispersing on to media contained in a dish. The whole process can be done in less than 30 minutes.
The good part about the system is that the method is easy, simple (some optimization required with regard to the appropriate amount of DNA and the type of program used for each cell type), sensitive, requires minimal expertise and no prior plating of cells to be transfected is needed. This in contrast to the alternative methods which are limited by the need to plate cells at a particular density 24-48 hours prior to transfection. In addition, these methods are labor and time intensive. Amaxa has nucleofection kits with optimized protocols available for most common cell types. As nucleic acids are delivered straight into the nucleus, nucleofected cells can be ready for analysis after 2–6 hrs of transfection.
Amaxa’s website also provides you with detail information and a list of easy to follow optimized protocols for a variety of cell types. The bottom line is that the Nucleofector II is the right choice for fast, easy and efficient transfection of hard to transfect cells.
System MSRP is - $22,150 (USD) for commercial and about $12,000 (USD) for academic use. Buy this tested system for a fraction of the cost today!
System was recently calibrated and is located at Altogen Labs laboratory.