High-Performance Liquid Chromatography (HPLC) is a high-resolution technique for separation and quantitation of range of small molecules, biomolecules, and peptides. Ultra-high performance (UHPLC) systems are capable of increased pressure and are designed for increased resolution and performance. Platform availability ranges from all-in-one to modular and scalable systems. Metrics to consider include: pressure capacity, flow rate control, method development options, and compatibility. Columns and consumables are always a cost consideration, as well as maintenance, service, and repair. When purchasing, consider whether a new system is backward compatible with existing methods and if software is available for used models.
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AKTA ExplorerAgilent 1100Agilent 1200 HPLCAgilent 1260 Infinity HPLCAgilent 1290Agilent HPLCAgilent Technologies 1200 Series Rapid ResolutionAgilent Technologies 1220 Infinity LC SystemAgilent Technologies 971-FPBeckman GoldBotanical Extraction EquipmentDionex Ultimate 3000
Eksigent nanoLCHP 1090HP 1100 HPLCHewlett Packard 1050Hitachi HPLCInMotionJASCO X-LC SystemsKnauer SmartlineLab Alliance Series 1500 SystemLachat Instruments QuikChem 8500 Series 2Perkin Elmer 200PerkinElmer Series 200Piston PumpProcess ChromatographyShimadzu HPLCShimadzu Prominence HPLCShimadzu UFLCSupercritical Fluid ExtractorThermo Scientific AccelaThermo Scientific UltiMate 3000 Basic ManualVarian 920-LC Analytical HPLCVarian PrepStar SD-1 and SD-2Waters 2690Waters 2695Waters AcquityWaters Acquity UPLCWaters AllianceWaters Alliance HPLC DissolutionWaters Breeze 2 HPLCWaters HPLCWaters Semi-Preparative to Preparative-Scale HPLC PurificationWaters UPLC