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The iWestern workflow is designed to address processing efficiency, reproducibility, and robustness, enabling exceptional western blot results with minimal hands-on time. Streamline your western blotting workflow by empowering your lab with the Invitrogen iWestern workflow solution.

The Invitrogen™ iWestern Workflow

Conventional western blotting is slow and inefficient.

Conventional western blotting is long, tedious, multi-step process that can take between 18-24 hours.

Sequential manual processing steps are required with attention needed at each step.
The multiple hands-on activities can introduce errors into the conventional workflow.
Errors can lead to inconsistency and decreased reproducibility across blots.

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Western Blotting Workflow Comparison

Traditional Workflow

The traditional western workflow requires extensive time and often produces inconsistent and elusive results.

iWestern Workflow

The iWestern workflow is an intuitive take on the decades-long problem, enabling exceptional western blot results with minimal hands-on time.

Step 1

Electrophoresis

Prepare, Load, and Run Gels

90

minutes

VS

50

minutes

with Invitrogen

Load and Run Precast Gels

Watch and Download

Download Protein Gel Guide (PDF)

Protein Gel Electrophoresis and Western Blotting Education Center (Web Resource)

The iWestern Workflow: Electrophoresis

Mini Gel Tank and PowerEase Touch Power Supply

Intelligently engineered side-by-side gel cassette design
Forward-facing well configuration for easier sample loading and visualization of both gels
White-colored tank stand provides contrast to improve the monitoring of pre-stained markers during electrophoresis
Touch screen LCD display for easier programming with clear menu prompts and view run progress.
Pre-programmed modes for Invitrogen precast protein gels & applications to help save time and minimize errors

The Invitrogen™ Mini Gel Tank is designed for more intuitive use and greater convenience compared to traditional electrophoresis tanks. Invitrogen™ PowerEase™ Touch Power Supplies bring a new level of convenience to your electrophoresis experiments, with a bright, LCD touch screen interface, and easy-to-use programing for a variety of applications.

Higher-throughput Solution: SureLock Tandem Midi Gel Tank

Load up to 26 samples in Invitrogen precast midi protein gels.
Separate gel chambers allowing use of only the necessary amount of buffer for each gel, minimizing buffer cost and waste.
Run or transfer up to 2 Invitrogen midi gels in the same tank at room temperature.

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Step 2

Transfer

Assemble, Prepare, and Run Transfer Device

75

minutes

VS

7

minutes

with Invitrogen

All-in-One Transfer Devices

Watch and Download

Take the iBlot 2 Virtual Tour

Protein Gel Electrophoresis and Western Blotting Education Center (Web Resource)

Download Western Blotting Overview Brochure (PDF)

The iWestern Workflow: Transfer

iBlot 2 Dry Blotting System

The short distance between electrodes, along with high field strength and current, reduces transfer time to 7 minutes
Innovative design uses pre-assembled transfer stacks that streamlines transfer setup and teardown
Transfer buffer is incorporated into the gel matrices; no need to prepare messy transfer buffers

The Invitrogen™ iBlot 2 Dry Blotting System is a true self-contained, plug-and-play system, with an integrated power supply and pre-packaged ready-to-use transfer stacks. The system has a simple touchscreen interface and pre-programmed optimized transfer programs, with the option to customize programs to suit your needs.

High-throughput Solution: Power Blotter Systems

Load up to 4 mini or 2 midi protein gels simultaneously
Integrated internal power supply designed for rapid 7-10 minutes transfers
Versatile system that can be used with build-it-yourself, ready-to-build or ready-to-use transfer stacks

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Step 3

Antibody Probe

Multiple hands-on antibody incubation and wash steps, followed by substrate reaction

875

minutes

VS

185

minutes

with Invitrogen

Automated antibody incubations and washes, followed by substrate reaction

Watch and Download

Take the iBind virtual tour (Web Resource)

Download Bandmate brochure (PDF)

The iWestern Workflow: Probe

iBind Western Systems

Sequential Lateral Flow (SLF) technology allows rapid processing of solutions
Blocking and antibody incubation solutions are optimized to work with SLF technology
Robust results with less primary antibody, equal to or better than manual processing methods

The Invitrogen™ iBind Western Systems are simple, non-powered devices that automate many of the tedious, routine western blot processing steps. The entire immunoblotting process is complete in less than 3 hours, uses up to 80% less primary antibody than traditional methods, and because processing is automated, blot-to-blot consistency can be improved.

High-throughput Solution: Bandmate Automated Western Blot Processor

Programmable blot rocking system that automates blocking, washing and antibody incubations
Process up to 4 mini blots or 2 midi blots using your current optimized reagents and protocols

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Step 4

Detection

X-ray film based detection

5

minutes

VS

2

minutes

with Invitrogen

CCD-camera based detection and imaging

Watch and Download

Download iBright Imaging Systems Brochure (PDF)

iBright Imaging System Resources (Videos/Webinars)

The iWestern Workflow: Detection

iBright Imaging Systems

Smart Exposure acquisition technology for rapid determination of optimal exposure time, combined with a sensitive 9.1 megapixel camera
Logical interface, automatic zoom, focus, and sample rotation simplify the image capture workflow*
All models offered with 21 CFR part 11 compliance support software packages to set up and control security, auditing, and e-signature settings

As Invitrogen™ iBright Imaging Systems capture the data in a digital format, this eliminates a scanning step required to process x-ray film for analysis. Perform molecular weight marker overlays, densitometry analysis, and normalization on-instrument or with our standalone iBright™ Analysis Software, available in both desktop and cloud based formats.

There are three models in the iBright Imaging Systems family: the iBright™ CL750 Imaging System, the iBright™ CL1500 Imaging System, and the iBright™ FL1500 Imaging System. The iBright CL750 Imaging System offers the core essential western blot and gel imaging functions and makes the transition from the darkroom and film easy. The iBright CL1500 Imaging System expands application support and has many of the high-performance specifications of our premier iBright FL1500 model. The iBright FL1500 Imaging System features maximum application support, including fluorescent western blot imaging with up to four fluorescence channels at a time.
‍

*Note: automatic rotation available only in iBright FL1500 and CL1500 models.

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Result

Total Time Spent

Tradtional Western
Blot Workflow

18

hrs

1,200 min

Save
up to

14

Hours

Invitrogen iWestern
Workflow

4

hrs

244 min

Learn more about iWestern workflow Contact a technical specialist

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Conventional western blotting is slow and inefficient.

Western Blotting Workflow Comparison

Traditional Workflow

iWestern Workflow

VS

Traditional Western Blot

Invitrogen iWestern Workflow

Step 1

Electrophoresis

Prepare, Load, and Run Gels

90

VS

50

Load and Run Precast Gels

Step 2

Transfer

Assemble, Prepare, and Run Transfer Device

75

VS

7

All-in-One Transfer Devices

Step 3

Antibody Probe

Multiple hands-on antibody incubation and wash steps, followed by substrate reaction

875

VS

185

Automated antibody incubations and washes, followed by substrate reaction

Step 4

Detection

X-ray film based detection

5

VS

2

CCD-camera based detection and imaging

Result

Total Time Spent

18

1,200 min

Saveup to

14

Hours

4

244 min

Save
up to