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ThermoFisher

The iWestern workflow is designed to address processing efficiency, reproducibility, and robustness, enabling exceptional western blot results with minimal hands-on time. Streamline your western blotting workflow by empowering your lab with the Invitrogen iWestern workflow solution.

iWestern workflow solution on table

The Invitrogen™ iWestern Workflow

Conventional western blotting is slow and inefficient.

Conventional western blotting is long, tedious, multi-step process that can take between 18-24 hours.

  • Sequential manual processing steps are required with attention needed at each step.
  • The multiple hands-on activities can introduce errors into the conventional workflow.
  • Errors can lead to inconsistency and decreased reproducibility across blots.

Western Blotting Workflow Comparison

Traditional Workflow

The traditional western workflow requires extensive time and often produces inconsistent and elusive results.

iWestern Workflow

The iWestern workflow is an intuitive take on the decades-long problem, enabling exceptional western blot results with minimal hands-on time.

VS

Traditional Western Blot

Invitrogen iWestern Workflow

Step 1

Electrophoresis

Load and Run Precast

Prepare, Load, and Run Gels

90

minutes

VS

50

minutes
with Invitrogen
Load and Run Precast

Load and Run Precast Gels

Step 2

Transfer

Load and Run Precast

Assemble, Prepare, and Run Transfer Device 

75

minutes

VS

7

minutes
with Invitrogen
Load and Run Precast

All-in-One Transfer Devices

Step 3

Antibody Probe

Load and Run Precast

Multiple hands-on antibody incubation and wash steps, followed by substrate reaction

875

minutes

VS

185

minutes
with Invitrogen
Load and Run Precast

Automated antibody incubations and washes, followed by substrate reaction

Step 4

Detection

Load and Run Precast

X-ray film based detection

5

minutes

VS

2

minutes
with Invitrogen
Load and Run Precast

CCD-camera based detection and imaging

Result

Total Time Spent

Tradtional Western
Blot Workflow
>

18

hrs

1,200 min

Save
up to

14

Hours

Invitrogen iWestern
Workflow
~

4

hrs

244 min