The CytoFLEX SRT 4 Laser V5-B2-Y5-R3 benchtop cell sorter integrates advanced technologies that simplify setup and operation, enabling researchers to focus on their scientific work. It features four active lasers (405 nm, 488 nm, 561 nm, 638 nm) and 15 active fluorescence detectors. The system is equipped with 15 repositionable bandpass filters: 450/45, 525/40 (x2), 585/42, 610/20 (x2), 660/10 (x2), 675/30, 690/50, 710/50, 712/25, and 780/60 (x3).

The CytoFLEX SRT in our inventory was manufactured in 2021.

Included in the complete system are a computer workstation preloaded with the necessary software, along with sheath and waste containers, all featuring a harness for easy operation.

The CytoFLEX SRT will be delivered certified, accompanied by a 90-day warranty.

For additional details, please feel free to contact us.

Specifications: (From Beckman)

General
Sorter	72.5 cm x 47.5 cm x 45 cm
Fluidics cart	34.5 cm x 60 cm x 48.5 cm
Weight
Sorter	62 kg
Fluidics cart	13.5 kg
Acoustic Noise Level	≤ 65 dBA
Electrical Requirement	100-240 VAC, 50-60 Hz
Optics
Excitation Optics	The CytoFLEX SRT system is configured with four spatially separated lasers. The optical system is alignment-free. The laser delays are automatically adjusted by the daily QC system, if required. No user intervention is required to ensure optimum system performance.
Laser devices	Violet laser: 405 nm, 90 mW Blue laser: 488 nm, 50 mW Yellow laser: 561 nm, 30 mW Red laser: 638 nm, 100 mW
Optical Filters	450/45, 525/40 (2), 585/42, 610/20 (2), 660/10 (2), 675/30, 690/50, 710/50, 712/25, 780/60 (3)
Forward scatter detection	Silicon photodiode with built-in 488/15 band-pass filter.
Fluorescence and side scatter detection	Fluorescence and side scatter light collected by the objective lens is delivered by fiber optics to a patent-pending design with high performance, solid-state, high efficiency, low-noise detector array. Reflective optics with a single transmission band-pass filter in front of each detector.
Violet side scatter configuration (VSSC)	The system offers the ability to configure the violet laser detector to collect side scatter to better resolve nanoparticles from noise.
Fluidics System
Sample flow rate	10~100 μL/min
Nozzle Dimension	100-μm internal diameter.
Fluid capacity	Sheath tank: 4-L / 7.5-L, Waste container: 7-L, Shutdown fluid container:1-L
Automated maintenance cycles	System Startup program, Backflush, Sheath Filter De-bubble, Flow Cell Debubble, Flow Cell Clean, Daily Clean, Aseptic Clean, System Shutdown program, Long-term shutdown.
Sample input formats	5 mL (12 x 75 mm) polystyrene and polypropylene sample tubes
Sample dead volume	< 60 μL
Supported sort collection devices
1-way sorting	6-well plates, 24-well plates, 48-well plates, standard 96-well plates, 96-well deep well plates, 384-well plates, slides
4-way sorting	4 x 5 mL tubes, 2 x 15 mL tubes and 2 x 5 mL tubes
2-way sorting	2 x 15 mL tubes
Electronics
Signal processing	7-decade data display
Signal	Pulse area and height for every channel, width for two selectable channels
Data Management
Software	CytExpert SRT software
Language	English and Chinese
FCS format	FCS 3.0
Performance
Sensitivity	FITC: < 30 molecules of equivalent soluble fluorochrome (MESF-FITC) PE: < 10 molecules of equivalent soluble fluorochrome (MESF-PE) APC: < 25 molecules of equivalent soluble fluorochrome (MESF-APC)
Fluorescence resolution	rCV < 3% The CytoFLEX SRT cell sorter is capable of achieving <3% rCV. Using CytoFLEX Daily QC Fluorospheres or CytoFLEX Ready to Use Daily QC Fluorospheres for daily QC, the pass criteria is ≤ 5%.
Side scatter resolution	< 300 nm
Violet Side scatter resolution	200 nm relative to polystyrene particles
Forward and side scatter resolution	≤ 1 μm Scatter performance is optimized for resolving lymphocytes, monocytes, and granulocytes as well as nanoparticles.
Carryover	< 0.1% (QC beads)
Sorting	The system shall be capable of simultaneously bulk sorting up to 4 defined populations of particles with a mode that allows ≥ 99% at purity mode with meeting the criteria below: • Target population ≤ 5% • Sample threshold rate ≤ 10,000 EPS • Yield rate: ≥ 80% theoretic rate An efficiency rate up to 30,000 events per second can be achieved without affecting purity but with a decrease in yield based on Poisson’s statistics.

Beckman Coulter Life Sciences

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