Unlocking ESR1 Mutation Detection with Droplet Digital PCR

Mutations in the ESR1 gene are a leading cause of resistance to endocrine therapy in estrogen receptor–positive breast cancer. Detecting these variants early is critical for advancing clinical research and developing strategies that improve patient outcomes.

The ddPLEX ESR1 Mutation Detection Kit from Bio-Rad enables precise, single-well detection of seven common ESR1 variants (E380Q, S463P, L536R, Y537C, Y537N, Y537S, and D538G) with unparalleled sensitivity. In this exclusive whitepaper, Bio-Rad scientists share new data on detection linearity, variant allele frequency (VAF) analysis, and workflow demonstrations using contrived plasma samples.

What You’ll Learn

• Why ESR1 mutations drive resistance in hormone receptor–positive breast cancer
• How the ddPLEX ESR1 Mutation Detection Kit delivers linear detection across a 0.05–50% VAF range
• Practical insights from experiments using plasma cfDNA and FFPE tissue samples
• How a streamlined workflow can support routine mutation monitoring in preclinical and clinical research studies

Why Download This Whitepaper?

• Stay ahead of resistance research – Learn how ESR1 monitoring can inform better study designs
• Evaluate performance data – See the linearity and sensitivity proven in Bio-Rad’s validation studies
• Support your lab workflows – Explore a practical, convenient approach for longitudinal ESR1 testing

Download the Whitepaper Today
Gain insights into how you can integrate the ddPLEX ESR1 Mutation Detection Kit into your research for more confident, reproducible results.