Unlock the Tools, Techniques, and Workflows Defining the Future of Spatial Biology

Spatial Imaging is revolutionizing how we visualize biological processes. Advances in multiplexed imaging, digital microscopy, and high-resolution spatial assays are rapidly transforming how researchers interrogate tissue architecture, cellular neighborhoods, and complex disease microenvironments. Yet with an expanding array of platforms and chemistries, developing a robust spatial workflow can be challenging.

This comprehensive eBook—Spatial Imaging Demystified—guides researchers through the core principles, instrumentation, and experimental considerations needed to generate reproducible, high-content spatial datasets that preserve biological context and support translational discovery.

What You’ll Learn:

Foundational Concepts in Spatial Biology: Understand how spatial transcriptomics, proteomics, genomics, and metabolomics integrate digital imaging with molecular profiling to map biological processes within intact tissues.

Fundamentals of Spatial Imaging and Multiplex Fluorescence:  Explore the scientific basis of preserving spatial context—co-localization, segmentation, multiplexing, and tissue-level structural analysis—and how these principles elevate discovery in immuno-oncology, neuroscience, and pathology.

Technique Comparisons: Spectral vs. Cyclic Imaging: Gain clarity on when to deploy mid-plex spectral imaging versus high-plex cyclic methods, including tradeoffs in throughput, sample integrity, data quality, and panel design.

Designing Robust Spatial Imaging Experiments: Learn best practices in specimen preparation, antibody validation, image acquisition, cell segmentation, and cross-batch harmonization to ensure reproducible single-cell spatial data.

Mid-Plex Imaging and the Evolution of Practical Spatial Workflows

Discover why many labs are shifting toward 5–9 marker mid-plex imaging—balancing biological complexity with operational efficiency—and how the EVOS™ S1000 Spatial Imaging System supports streamlined, single-scan multiplex analysis.

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Get your copy of Spatial Imaging Demystified and bring clarity, confidence, and technical rigor to your spatial biology workflows.