Affordable and user-friendly image splitter that enables two-camera, simultaneous dual-emission imaging from a single microscope port

Easy to align and maintain
Ideal for FRET and wide-field large sensor simultaneous dual-channel imaging

The TwinCam LS Image Splitter has been designed as a convenient solution for simultaneous two-channel imaging without sacrificing field of view. Traditionally, multi-wavelength imaging experiments required that multiple images be captured sequentially. However, because the various images were not taken at the same time, temporal artifacts, caused by intracellular movement, often appeared between exposures. To eliminate these temporal artifacts and provide the most accurate measurements, the TwinCam LS allows two images to be acquired simultaneously.

This image splitter enables pixel alignment between two cameras coupled to a single microscope port. The base unit accommodates a standard Cairn filter cube allowing light to be distributed on the basis of wavelength, polarization state, or focal depth. Each cube has fine mechanical X-Y adjustment for pixel overlay or deliberate image offset. The input of the instrument has a variable rectangular aperture enabling the use of cropped sensor mode on both detectors.

Applications:

Förster Resonance Energy Transfer (FRET)
Simultaneous use of multiple dyes or genetic markers
Ratiometric imaging
Polarization studies
Simultaneous transmitted light and fluorescence imaging
Simultaneous high speed and high resolution imaging
Multifocal Plane Microscopy

Specifications:

Standard spectral range: 450 nm – 900 nm
Dimensions: 348 mm (L) x 191.5 mm (W) x 72 mm (H)
Weight: 1.8 kg
Emission filter size: 25 mm
Dichroic mirror size:
25.5 mm x 36.0 mm OR 26.0 mm x 38.0 mm
up to 3.0 mm thick, UltraFlat dichroics highly recommended

COSA Instrument Corp.

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