BIO RAD QX200 AutoDG Droplet Digital PCR System

**This unit has been tested to specifications. Has slight cosmetic blemishes from normal use. Software not included. Buyer can expect same unit as pictures. See photos for further details

The QX200 AutoDG Droplet Digital PCR System provides absolute quantification of target DNA or RNA molecules for EvaGreen and probe-based Droplet Digital PCR (ddPCR) applications. The Automated Droplet Generator simplifies the ddPCR workflow, making digital PCR both scalable and practical. The AutoDG Instrument eliminates user-to-user variability, allowing you to fully exploit the precision of ddPCR technology by providing more consistent droplet counts across users and plates. The AutoDG Instrument also minimizes hands-on time — it takes less than 5 minutes to load the instrument, and no supervision or user activity is required during droplet generation.

Features and Benefits of the QX200 AutoDG Droplet Digital PCR System

Automated Droplet Generator — simplifies the ddPCR workflow and offers flexibility:

Generates droplets for 96 ddPCR reactions in under 45 minutes

Compatible with both the QX100 and QX200 Droplet Digital PCR Systems

A single AutoDG Instrument can supply 4–5 Droplet Readers continuously

Guides setup and loading with large, color touch-screen interface and LED lighting

User-definable plate layout with increments of 8 wells provides run-to-run flexibility

HEPA-filtered enclosure reduces the possibility of contamination and allows the system to be placed in any lab environment

Droplet Digital PCR Technology — the most precise and sensitive digital PCR solution for a wide variety of applications, providing:

Flexible digital PCR chemistry — optimized for TaqMan Hydrolysis Probe and EvaGreen Assays

Nanoliter droplet partitioning — reduces bias from amplification efficiency differences and PCR inhibitors

Convenient assay design — standard curves are not required

Applications of the QX200 Droplet Digital PCR System

Cancer biomarker studies and copy number variation — measure varying degrees of cancer mutations, detect rare DNA target copies, and resolve copy number variation states with superior sensitivity and resolution. Predesigned, wet-lab validated PrimePCR ddPCR Assays are now available for mutation and copy number detection.

Pathogen detection — employ the extreme precision of the QX200 System to quantify small fold changes in target DNA or RNA molecules in pathogen detection and monitoring

Next generation sequencing — perform absolute quantification of an NGS library without the use of a standard curve

Gene expression analysis — achieve reliable and reproducible measurements of small fold changes for low-abundance mRNA and miRNA

Environmental monitoring — test a wide variety of environmental samples such as soil and water

Food testing — perform routine evaluation of genetically modified organisms (GMO) using validated ddPCR methods

The QX200 AutoDG Droplet Digital PCR System requires the use of the PTC Tempo Deepwell Thermal Cycler or C1000 Touch Thermal Cycler with 96–Deep Well Reaction Module and the PX1 PCR Plate Sealer.

Required Consumables

DG32 Cartridges for AutoDG

Automated Droplet Generation Oil for Probes

Automated Droplet Generation Oil for EvaGreen

Pipet Tips for AutoDG

Pipet Tip Waste Bin for AutoDG

Droplet Reader Oil or ddPCR Droplet Reader Oil EcoTank for Probes

ddPCR Reagents for EvaGreen or TaqMan Hydrolysis Probe-Based Applications

PrimePCR ddPCR Assays

ddPCR Supermixes for Probes (no dUTP)

QX200 ddPCR EvaGreen Supermix

ddPCR Supermix for Probes

One-Step RT-ddPCR Kit for Probes

The AutoDG Instrument partitions each sample in a 96-well PCR plate into ~20,000 uniform nanoliter-sized droplets in which nucleic acid molecules are randomly distributed. Every droplet serves as an individual PCR reaction in Bio-Rad’s Droplet Digital (ddPCR) PCR System. After partitioning, the plate is sealed with the PX1 Plate Sealer, and PCR is run to end point in a thermal cycler. The droplets in each sample can then be analyzed using a QX600 or QX200 Droplet Reader.

Eliminates user-to-user variability — creates more consistent droplet counts across users and plates, enabling you to fully exploit the precision of your ddPCR System

Minimizes hands-on time — takes less than 5 minutes to load the instrument, and droplet generation requires no supervision

Features and Benefits of the Automated Droplet Generator

Bio-Rad’s Automated Droplet Generator simplifies ddPCR workflow and makes digital PCR scalable and flexible:

Guides setup and loading with large, color touch-screen interface and LED lighting

Generates droplets for 96 ddPCR reactions in under 45 minutes

One AutoDG Instrument can generate sufficient droplets to keep 4–5 droplet readers running continuously

Provides operational flexibility:

User-definable plate layout with increments of 8 wells

HEPA-filtered enclosure reduces the possibility of contamination and allows the system to be placed in any lab environment

Compatible with both QX600 and QX200 ddPCR workflows

Specifications

Starting sample size, µl	20
Automated Droplet Generator capacity	1–96 samples/run
Droplets per 20 µl sample	20,000
QX200 Droplet Reader capacity	1–96 samples
Sample illumination	Light-emitting diodes
Sample detection	Multi-pixel photon counter
Detection channels	FAM (EvaGreen), HEX (VIC)
Linear dynamic range	5 orders of magnitude
Precision	±10%
Droplets per 96-well plate, million	~1.5

Digital PCR (dPCR)

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