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18-angle light scattering detector for the measurement of absolute molecular weight, size, and conformation of macromolecules in solution. The DAWN® HELEOS™ II may be used in batch mode (off-line) or connected on-line to an HPLC/HPSEC/AFFF, etc.. The DAWN® HELEOS™ II utilizes a 120mW solid-state laser operating at 658 nm and also has on-board digital signal processing hardware for up to four external devices such as RI and/or UV detectors. Also contains an analog output from the 90° detector for interfacing to strip chart recorders. The DAWN® HELEOS™ II has a gorgeous 64,000 color LCD display, thermostatic control options, and depolarization options.