Fluorescence Microscope
Illumination is typically performed using a xenon arc lamp, mercury-vapor lamp, LED, or laser, making selection and consideration of cost and performance important.
Other considerations include: the quality and durability of the objectives, the quality of components such as the spectral emissions filter, the focus and stage manipulation features, as well as the image capture system and associated specifications.
Fluorescence microscopy is an essential tool in the biological and biomedical sciences, as well as other fields, owing to attributes over and above those in available with traditional light microscopy.
The technique involves illumination with fluorescent or phosphorescent light of specific wavelengths matching absorption by fluorophores in the sample, thereby causing them to emit light back at different wavelengths. These fluorophores can be intrinsic to the sample or added extrinsically from commercial sources.
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