This compact and powerful 6-laser flow cytometer isolates and analyzes blood or cultured cells, bacteria, algae, and micro- and nanoparticles. It reliably detects 80 nm polystyrene beads and offers exceptional sensitivity and reproducibility, with six excitation wavelengths: 355 nm (UV), 405 nm (Violet), 488 nm (Blue), 561 nm (Yellow-Green), 638 nm (Red), and 808 nm (IR).

The CytoFlex LX in our stock was manufactured in 2019.

Our CytoFlex LX model is fully equipped with all six lasers and provides the maximum optical power and capabilities for this model: (U3-V5-B3-Y5-R3-I2).

This system will be certified and go with a 90-day warranty.

A computer preloaded with the required software will also be included.

For more information, please feel free to contact us.

Specifications: (From Beckman Coulter)

General
Instrument dimensions (Length x Width x Height)
Cytometer	73.3 cm x 60.5 cm x 45.1 cm
Fluid Cubitainers	25 cm x 25 cm x25 cm
Weight
Cytometer [Without Plate Loader]	79 kg
Cytometer [With Plate Loader DW]	84 kg
Maximum Altitude	2000 m (6561 ft)
Pollution Degree	2
Acoustic Noise Level	<65 dBA
Electrical Ratings	100-240 VAC, 50/60 Hz, 250 W
Optics
Excitation optics	The optical system is alignment free. The laser delays are automatically adjusted by the daily QC system, if required. No user intervention is required to ensure optimum system performance. The LX system can be configured with up to six spatially-separated lasers.
Emission optics	Patent-pending alignment-free integrated optics quartz flow cell design with >1.3 NA. Flow Cell dimensions: 430 μm x 180 μm internal diameter.
Laser devices	Blue laser • Wavelength: 488 nm, 50 mW • Beam spot size: 5 μm x 80 μm Red laser • Wavelength: 638 nm, 50 mW • Beam spot size: 5 μm x 80 μm Violet laser • Wavelength: 405 nm, 80 mW • Beam spot size: 5 μm x 80 μm Yellow laser • Wavelength: 561 nm, 30 mW • Beam spot size: 5 μm x 80 μm Ultraviolet (UV) laser • Wavelength: 355 nm, 20 mW • Beam spot size: 5 μm x 80 μm Infrared (IR) laser • Wavelength: 808 nm, 60 mW • Beam spot size: 5 μm x 80 μm
Forward scatter detection	Silicon photodiode with built-in 488/8 band-pass filter.
Fluorescence and side scatter detection	Fluorescence and side scatter light collected by the objective lens is delivered by fiber optics to a patent-pending design with high performance, solid-state, high efficiency, low-noise detector array. Reflective optics with a single transmission band-pass filter in front of each detector.
Violet side scatter configuration (VSSC)	The system offers the ability to configure the violet laser WDM to collect side scatter to better resolve nanoparticles from noise.
Fluidics System
Sample flow rates
Fixed Flow Rates	10 μL/min, 30 μL/min, 60 μL/min
Custom Flow Rates	10-240 μL/min in 1 μL increments
CytoFLEX LX Specified	Standard 10L sheath fluid and waste cubitainers
Automated maintenance cycles	Initialize, system startup program, sample mix, backflush, prime, Daily Clean, Deep Clean
Single Tube Loader
Sample input formats	5 mL (12 x 75 mm) polystyrene and polypropylene sample tubes 1.5 mL and 2 mL micro-centrifuge sample tubes
Dead Volume	10 μL
Minimum Sample Volume	100 μL
Plate Loader DW
Sample input formats	96-well deep well plate Flat/U/V bottom standard 96-well plate
Dead volume
96-well deep well plate	20 μL
Flat bottom standard 96-well plate	20 μL
U/V bottom standard 96-well plate	10 μL
Minimum sample volume
Standard 96-well plate	50 μL/well
Deep well plate	200 μL/well
Maximum sample volume	20 μL
Data Management
Software	CytExpert software
Language	English and Chinese
FCS format	FCS 3.0
Operating system	Windows10 professional 64bit or Windows10 Enterprise LTSC 2019 64bit
Minimum workstation / computer requirements
Processor	4th Generation Intel Core i7 (8MB cache 4.0 GHz)
Memory	8 GB RAM
Storage	500 GB
Ethernet	Integrated 10M/100M/1000M Gigabyte
USB	4 USB 2.0 and above ports
Performance
Sensitivity	FITC < 30 MESF PE < 10 MESF APC < 25 MESF
Fluorescence resolution rCV	<3%
Blue side scatter resolution	<300 nm
Violet side scatter resolution	80 nm relative to polystyrene particles
Forward and side scatter resolution	Scatter performance is optimized for resolving lymphocytes, monocytes, and granulocytes as well as nanoparticles
Carryover (Single Tube)	≤1.0%
Signal acquisition speed (Single Tube)	30,000 particles/second with 23 parameters
Carryover (Plate)	≤1.0%
Throughput of Std 96W Plate (10 second acq wo/ mixing or backflush)	<39 min
Throughput of Deep 96W Plate (10 second acq wo/ mixing or backflush)	<40 min
Throughput of Std 96W Plate (10 sec acq, 5 sec mixing, 6 sec backflush)	<60 min
Throughput of Deep 96W Plate (10 sec acq, 5 sec mixing, 6 sec backflush)	<69 min

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