Applied Biosystems QuantStudio 12K Flex Real-Time PCR System w/ 96-Well Fast Sample Block

Block Configuration

96-Well Fast (15 to 30 uL reactions)

Maximum Ramp Rate

3.0°C/second

Well-to-Well Variability

± 0.25°C

Features

Processes up to 110,000 data points in an 8-hour day with minimal hands-on time

Five interchangeable block formats for maximum workflow flexibility

Compatible with automation systems like the Thermo Scientific Orbitor RS2 Microplate Mover

Enhanced OptiFlex system enables multiplex detection of up to six targets

Supports 21 CFR Part 11 compliance with integrated security and audit features

Rapid block changes in under one minute without the need for tools

High sensitivity detection down to a single copy with a dynamic range of nine logarithmic units

Ideal for diverse applications such as drug discovery, pharmacogenomics, and agricultural testing

Digital PCR capability for precise detection of rare genetic targets

Streamlined workflow with just 20 minutes of hands-on time per run

The Applied Biosystems QuantStudio 12K Flex Real-Time PCR System w/ 96-Well Fast Sample Block is a high-throughput, versatile platform designed to meet diverse laboratory needs in gene expression, genotyping, and various PCR applications. This system offers unmatched scalability and efficiency, capable of processing up to four 3,072-reaction OpenArray plates in approximately four hours, producing up to 110,000 data points within an eight-hour workday when paired with the QuantStudio 12K Flex OpenArray AccuFill System. With just 20 minutes of hands-on time per run, it maximizes productivity while minimizing labor.

Engineered for flexibility, the QuantStudio 12K Flex features five interchangeable block formats - OpenArray plate, TaqMan Array Card, 384-well, and both 0.1 mL and 0.2 mL 96-well blocks - allowing seamless transitions between workflows without the need for tools. The instrument supports high-throughput environments with laboratory information management system (LIMS) compatibility and built-in features that facilitate 21 CFR Part 11 compliance, including integrated sample tracking, security, auditing, and electronic signature (SAE) modules for enhanced data integrity and quality control.

Advanced OptiFlex technology with six independent excitation and emission filter channels enables superior fluorescence detection and multiplexing, supporting up to six targets per reaction. This adaptability makes the system ideal for applications such as drug discovery, pharmacogenomics research, microRNA profiling, agricultural molecular testing, CFTR mutation analysis, and vaginal microbiota studies. The QuantStudio 12K Flex system is automation-compatible, integrating seamlessly with the Thermo Scientific Orbitor RS2 Microplate Mover to streamline workflows and reduce manual handling. It also supports digital PCR with QuantStudio digital PCR kits and DigitalSuite Software, enabling precise detection of rare targets, including cancer mutations and viruses.

Built for speed and accuracy, the system offers a maximum block ramp rate of 3.0°C/second, well-to-well temperature variability of ± 0.25°C, and demonstrated sensitivity down to a single copy. The enhanced OptiFlex system utilizes a white LED excitation source and decoupled detection channels for high-resolution results with dynamic range spanning nine logarithmic units and singleplex resolution as low as a 1.5-fold change. Designed for both current and future research demands, the QuantStudio 12K Flex Real-Time PCR System delivers unparalleled throughput, flexibility, and precision - empowering laboratories to expand the boundaries of their research with confidence. Please Note: We recommend performing a Dye Calibration for the Dye you plan on using for your application(s). Please refer to the Operator's Manual for instructions.

All of our equipment is fully tested to perform at factory specifications before being shipped.

INCLUDED:

The Applied Biosystems QuantStudio 12K Flex Real-Time PCR System w/ 96-Well Fast Sample Block includes:

> 96-Well Fast Block

> 96-Well Fast Heated Cover

> Background Calibration Report

> ROI Calibration Report

> Uniformity Calibration Report

> Windows 11 Professional 64-Bit Data Station with Flatscreen Monitor

> QuantStudio 12K Flex Software Installed

> Power Cord

> Operator's Manual

> Full 180 Day Parts and Labor Warranty

Applied Biosystems QuantStudio 12K Flex Real-Time PCR System w/ 96-Well Fast Sample Block Specifications

Block Configuration:	96-Well Fast (15 to 30 uL reactions)
Block Change Design:	Block change from front in less than 1 minute; no tools required
Maximum Block Ramp Rate:	3.0°C/second
Well-to-Well Variability:	± 0.25°C
Run Time:	30 minutes expected (96-Well Fast block)
Excitation Source:	Enhanced OptiFlex system, white LED
Detection Channels:	Decoupled: 6 emission, 6 excitation
Demonstrated Sensitivity:	To 1 copy
Dynamic Range:	To 9 logarithmic units
Resolution:	As low as 1.5-fold change for singleplex reaction
Remote Monitoring:	Available to monitor up to 15 networked instruments simultaneously
Dimensions (H x W x D):	29.1 x 19.7 x 26 in. (73.8 x 50.1 x 66.1 cm)
Weight:	154.3 lb. (70 kg)
Electrical Requirements:	100-240V, 50/60Hz, 12.5A

Computer Specifications
Processor:	Intel Core I5-8500 CPU
RAM:	32.0 GB
Hard Drive:	500GB Internal SSD
Operating System:	Windows 11 Professional, 64-bit

Thermal Cycler Inspection and Recertification Checklist

Validate that the power supply and PCB consistently deliver accurate, stable voltages across all circuits and connections, confirming operational reliability

Thoroughly inspect the display for any dim, flickering, or dead pixels, replacing compromised segments to ensure clear visibility and precise data display

Verify complete functionality and responsiveness of all buttons and knobs, confirming accurate and reliable input across all controls

Ensure the machine completes all internal diagnostic checks with zero errors, indicating system readiness and functional integrity

Confirm the machine accurately identifies attached blocks (96-well, dual 48, etc.), with seamless recognition across different configurations

Conduct a detailed inspection of internal wiring and all electrical connections, checking for any signs of breaks, disconnections, or tears; replace or repair any underperforming components

Examine the connector between the block and base for bent or broken pins, replacing as needed to maintain secure, reliable connections

Calibrate well temperatures with a NIST-traceable thermometer, verifying accuracy and uniformity across all wells to meet specification standards

Perform a comprehensive cosmetic inspection, ensuring all exterior surfaces and components are free of damage, wear, or defects, maintaining aesthetic and professional quality

Technician Inspection and Recertification Checklist: Thermal Cycler

The Following Checklist Is to Be Performed on All Incoming and Outgoing Pieces of Equipment

Task:	Completed?:	Remarks?
Validate stable, accurate voltage outputs from power supply and PCB across circuits:	Completed:
Inspect display for dim, flickering, or dead pixels; replace as needed for clarity:	Completed:
Verify functionality and responsiveness of all buttons and knobs:	Completed:
Confirm machine completes internal diagnostics with zero errors:	Completed:
Ensure accurate block identification across configurations (96-well, dual 48, etc.):	Completed:
Inspect internal wiring and connections for integrity; repair or replace as needed:	Completed:
Check connector between block and base for bent or broken pins; replace as necessary:	Completed:
Calibrate well temperatures with a NIST-traceable thermometer for accuracy:	Completed:
Conduct cosmetic inspection to ensure quality and professionalism of all surfaces:	Completed:

To View More of Our Recertification Protocol Click Here

Downloads

Technical Specification Download

Operator's Manual Download

Typically Ships Within: 3 to 5 Business Days

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