Advances in Absorbance and Fluorescence Quantification – DNA, RNA, and Protein

Accurate quantification of DNA, RNA, and protein is essential for reliable downstream experiments. Absorbance and fluorescence spectroscopy provide complementary approaches to assess biomolecule concentration and purity.

Modern instruments, including the Thermo Scientific NanoDrop Ultra and Invitrogen Qubit 4, enable rapid microvolume measurements, contaminant detection, and actionable quality insights. NanoDrop Ultra combines absorbance and fluorescence in a single platform, while Qubit 4 fluorometers deliver precise quantification and RNA integrity assessment through intuitive assays.

 Key Learning Objectives:

• Compare absorbance and fluorescence for biomolecule analysis

• Understand contaminant identification for experimental success

• Review Qubit assays and their applications

• Learn how NanoDrop Ultra FL instruments measure absorbance and fluorescence in microvolume samples

Speakers

Patrick Brown, Senior Manager, Product Management 

Thermo Fisher Scientific  

Patrick Brown graduated with a masters in biomedical science from the Pennsylvania State University and an MBA from University of Delaware. He now leads the UV-Vis Product Management team for Thermo Fisher Scientific. In this role, he works with his team to collect user feedback, integrate feedback into software and hardware updates, and works with marketing teams to communicate these benefits to the scientists that trust Thermo Fisher Scientific instruments. 

Sneh Ringwala, Sr. Product Manager 

Thermo Fisher Scientific 

Sneh Ringwala is the product management leader for Qubit instruments and assays at Thermo Fisher Scientific. He partners closely with customers and internal teams to develop new products, training programs, and support tools that advance molecular biology research. With a focus on user-centered innovation, Sneh works to ensure Qubit continues to meet the evolving needs of scientists around the world.