High-Throughput CIM Monolith Format for AAV Process Development

Developing an efficient purification process for adeno-associated viral vectors (AAVs) is crucial for the success of new drugs during the investigational phase and throughout the product development lifecycle. Optimizing the AAV purification process early on can significantly reduce costs for clients and patients once large-scale production is established. We will present screening for the separation of empty and full AAVs using three different CIM QA HR formats and explain how we can scale them to process scale.

Key Learning Objectives:

1. Understanding the superiority of monoliths for purifying large biomolecules.
2. Exploring the use of different CIM monolithic plates in AAV process development.
3. Achieving high reproducibility across various product families and purification scales with the CIM QA HR line.
4. Guiding the selection of an appropriate CIM QA HR monolith product for screening based on specific laboratory needs and available equipment.

Speakers:

Marko Narobe, Product Manager for High-Throughput Solutions at Sartorius BIA Separations

Sara Drmota Prebil, PhD, Process Development Manager at Sartorius BIA Separations

Nicholas Pearman, PhD, Senior Scientist at Pharmaron Biologics UK Ltd.