Performance upgrades to a robust and proven platform

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BioTek is an industry leader in life science instrumentation, including liquid handling, multi-mode microplate detection, imaging and microscopy, and automation designed to serve a wide range of applications. BioTek products enable research by providing high-performance, cost-effective solutions to challenging tasks in many application workflows.

BioTek’s innovative microplate detection instruments excel in versatility, performance, and value. Available as multi-mode and specialized single mode systems, BioTek absorbance readers provide total flexibility for many applications from basic ELISA to high throughput detection.

Released in 1995, the ELx800 was a cost-effective, high quality absorbance reader line designed for applications within the clinical, biotechnology research and pharmaceutical laboratory. Its compact footprint and proven robust design made it an ideal solution for many microplate-based biological assays.

BioTek’s modern upgrade to this line, the 800 TS series, incorporates core features and significant enhancements over the older technology. Advancements include: a new color touchscreen interface, improved read speed, temperature and shaking control, onboard data processing, updated data ports, and optional printer compatibility.

Standard performance features built into the 800 TS.

  • The 800 TS platform is compatible with 6-, 12-, 24-, 48-, 96-, and 384-well microplates.
  • The available Gen5 software permits endpoint, kinetic, and area scanning modes for a variety of detection and imaging applications.
  • The Gen5 Reader Control software controls detector function and data collection while the optional Gen5 Data Analysis software allows computer based data analysis.

A wide wavelength detection range is coupled with excellent accuracy and precision.

  • Five optical filters are available and the Tungsten halogen light source and photodiode detector provide excellent dynamic range (0 to 3.0 OD) and resolution (0.001 OD).
  • The accuracy (<1% at 2.0 OD), linearity (<1% at 2.0 OD, <3% at 3.0 OD), and repeatability (<0.5% at 2.0 OD) together provide the high sensitivity levels needed to meet demanding applications.
  • This proven platform has produced years of optical performance with very low maintenance.

Beyond the platform’s robust design and optical performance, the new 800 TS line of instruments incorporate significant enhancements and performance capabilities.

Starting with upgrades in speed, the 800 TS adds significant improvements over the ELx800 for both single and dual wavelength detection.

  • In Normal mode, the 800 TS processes 96-well plates in 39 sec (35 sec kinetic) using single wavelength detection and 73 sec (69 sec kinetic) using dual wavelength detection. This is compared to ELx800 times of 50 sec and 95 sec, respectively.
  • In Rapid mode, the 800 TS single wavelength time of 26 sec (22 sec kinetic) is an improvement over 30 sec for the ELx800.
  • The 800 TS offers a new Sweep mode in addition to the Normal and Rapid modes, with speeds clocking in at 18 sec and 16 sec for kinetic measurements.

The user interface and data access features have been upgraded.

  • The new 800 TS incorporates a modern color LCD touchscreen, a stark departure from the two-line LCD touchpad in the older ELx800.
  • Data ports have been updated to 3 USB ports in the 800 TS, from RS232 (1), USB (1), and parallel port (1) seen previously on the ELx800.
  • There is also an optional Seiko RP-D10 USB printer for easy data readouts.
  • The 800 TS has delta and blanking capabilities built in. The LCD Touchscreen allows users to easily view and manipulate control of the 800 TS. User defined protocols can be run directly from the main menu.
  • The pre-configured and expanded Quick method now permits quick read, shake, or incubation options outside of a defined protocol.
  • The Protocol method allows users to define up to two wavelengths, plate type, read speed, shaking, temperature, and blank well locations.
  • Automated delta calculation and blank subtraction is possible.
  • The updated Results method permits viewing of the last 12 reads of 96 data points shown directly on the screen, with output options via USB and/or printer.
  • Finally, the Instrument method displays parameters, output options, utilities, data format, system test, absorbance test plate, and other information.

The 800 TS also includes optional shaking and temperature-controlled incubation modes, especially useful in temperature sensitive and kinetic measurements.

The devices are CE and TUV marked, are ROHS compliant, and IVD compliant. Many of the aforementioned modules and options are model specific, with three versions available for custom applications. Five 800 TS configurations are offered with model specific options and a number of available accessories.

Major upgrades in the 800 TS line of instruments such as detection speed, temperature control, and shaking, capabilities add significant value in applications ranging from endpoint ELISA assays to temperature-sensitive enzyme kinetic assays. The updated onboard software and LCD touchscreen capabilities provide a more complete interface with which to collect and process data. These attributes, combined with modern data access and output options, equate to a more versatile and complete solution for microplate absorbance detection.

This article was written by LabX and published in conjunction with BioTek.

BioTek Instruments, Inc., headquartered in Winooski, VT, USA, is worldwide leader in the design, manufacture, and distribution of innovative life science instrumentation.

We are the only life science instrumentation company with corporate headquarters, manufacturing, research and development, applications and service in the USA. Our company-wide commitment to quality and value is backed by our superior customer care, technical service centers, scientific application experts and a knowledgeable field sales and service specialists. Our expertise, quest for innovation, and efficiency all combine to provide the best possible technologies for our customers.

BioTek is ISO9001/ISO13485 certified, is an FDA Registered Medical Device Manufacturer, and has appropriate products in compliance with the EU In Vitro Diagnostic Directive (IVDD). Our quality program extends to your laboratory as well. BioTek offers optional validation (IQ/OQ/PQ) and FDA 21 CFR Part 11 tools to ensure regulatory compliance.