Polymerase Chain Reaction (PCR) is a ubiquitous technique used in molecular biology to amplify copies of DNA from template DNA. PCR is indispensable for genetic sequencing, molecular cloning, and genetic mutation analysis in human diagnostics. Thermo Cycler instruments are used to cycle PCR through the amplification process typically involving annealing, extension, and melting. Many devices are available according to scale, complexity, and application. Quantitative PCR (qPCR) provides quantitative data based on reference standards, Digital PCR (dPCR) provides highly sensitive quantitation without standards, and digital droplet PCR (ddPCR) uses massive sample partitioning for high-throughput data generation.
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