Best Microplate Readers of 2025: Top Picks for Research, Clinical & Industry Labs

ImageFX (2025)

In the dynamic world of scientific research and clinical diagnostics, the efficiency and accuracy of laboratory equipment are paramount. Labs constantly face the challenge of acquiring cutting-edge technology while adhering to stringent budgetary constraints. Among the most ubiquitous and indispensable tools in modern laboratories are microplate readers, workhorses that enable high-throughput analysis across diverse applications from drug discovery to academic research.

Microplate readers are sophisticated spectrophotometers designed to analyze multiple samples simultaneously in multi-well plates, ranging from 6 to an impressive 1,536 wells. Their ability to rapidly process numerous samples makes them critical for increasing productivity and accelerating scientific breakthroughs. Whether you're conducting ELISAs, protein quantification, cell-based assays, or complex kinetic studies, the right microplate reader can significantly impact your lab's capabilities. Fortunately, LabX offers an extensive selection of new, used, and refurbished microplate readers from trusted sellers, providing flexible options to suit every lab's needs and budget.

Quick List Summary: Top Microplate Readers

• Molecular Devices SpectraMax ABS Plus: Best for routine absorbance assays and robust performance.

• BMG LABTECH Spectrostar Nano: Ideal for dedicated high-speed absorbance measurements with high-throughput potential.

• Revvity VICTOR Nivo: Excellent for multi-mode functionality in academic or low-throughput research labs.

• Agilent BioTek Cytation 5: The ultimate solution for high-throughput, multi-mode detection combined with advanced imaging.

Molecular Devices SpectraMax ABS Plus

Best for: Routine absorbance assays and labs requiring a reliable workhorse for fundamental biochemical applications. Ideal for academic labs, quality control, and general research.

Price Range: ~$16,500 (used)

Why we picked it: The SpectraMax ABS Plus is a staple in many labs due to its proven reliability, ease of use, and versatility for standard absorbance applications. It offers a solid balance of performance and affordability, making it a go-to for everyday tasks.

Key specs and standout features:

• Detection Mode: Single-mode absorbance (UV and Visible spectra).

• Wavelength Range: 190-1,000 nm.

• Plate Compatibility: 96 to 384 well plates.

• Speed: Reads a 96-well plate in as little as 29 seconds.

• Integration: Compatible with microplate stackers and barcode readers for increased throughput and automation.

• Applications: Perfect for ELISAs, protein quantification (Bradford, BCA), nucleic acid quantification, and enzyme kinetics.

Pros:

• Reliable and robust for daily use.

• Wide wavelength range covers common assays.

• Automation-ready for higher throughput needs.

• User-friendly software.

Who it’s ideal for: Labs primarily focused on absorbance-based assays, those needing a dependable and affordable instrument for routine tasks, and facilities looking for a scalable solution for future automation.

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BMG LABTECH Spectrostar Nano

Best for: Labs dedicated to high-speed absorbance assays, particularly those with mid to high-throughput demands for single-mode applications.

Price Range: <$9,000 (refurbished)

Why we picked it: The Spectrostar Nano stands out for its exceptional reading speed for full UV/Vis spectra, making it incredibly efficient for absorbance-only applications. Its cost-effectiveness for a high-performance single-mode reader is a significant advantage.

Key specs and standout features:

• Detection Mode: Single-mode absorbance (full UV and Visible spectrum).

• Speed: Captures a full spectrum in 1 second per well.

• Plate Compatibility: Up to 1,536 well plates.

• Optics: High-performance optics optimized for absorbance.

• Compact Design: Space-saving footprint.

Pros:

• Extremely fast full-spectrum reading.

• High-throughput capability for absorbance.

• Cost-effective, especially for refurbished models.

• Ideal for dedicated absorbance workflows.

Who it’s ideal for: Academic labs, core facilities, or industrial labs where absorbance measurements are the primary focus and high-speed processing is crucial. It's a great choice for those who don't require multi-mode functionality but need robust absorbance performance.

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Revvity VICTOR Nivo Microplate Reader

Best for: Academic and research labs seeking a compact, affordable multi-mode reader for everyday biochemical and cell-based research with low to mid-throughput requirements.

Price Range: ~$10,000 (used)

Why we picked it: The VICTOR Nivo offers an impressive array of multi-mode capabilities in a remarkably small and affordable package. Its user-friendly software and remote access features make it particularly well-suited for multi-user lab environments.

Key specs and standout features:

• Detection Modes: Multi-mode (capable of measuring at almost any relevant wavelength, including absorbance, fluorescence intensity, luminescence, etc.).

• Size: One of the smallest multi-modal plate readers available.

• Software: Easy-to-use software with remote access.

• Applications: Designed for routine biochemical and cell-based assays.

Pros:

• Compact footprint saves valuable bench space.

• Affordable entry into multi-mode capabilities.

• Excellent for multi-user environments due to remote access.

• Versatile for a range of common lab assays.

Who it’s ideal for: Smaller academic labs, teaching labs, or research groups with diverse assay needs but limited space and budget. It's perfect for those transitioning from single-mode readers to more versatile platforms without a significant investment.

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Agilent BioTek Cytation 5 Microplate Reader

Best for: High-throughput research facilities, drug discovery labs, and core facilities requiring comprehensive multi-mode detection combined with advanced automated microscopy.

Price Range: >$70,000 (used)

Why we picked it: The BioTek Cytation 5 is a true powerhouse, integrating both microplate reading and automated microscopy into a single, highly configurable platform. Its extensive range of detection modes and imaging capabilities makes it incredibly versatile for complex and specialized applications.

Key specs and standout features:

• Detection Modes: Full range of multi-mode detection, including absorbance, fluorescence intensity, luminescence, time-resolved fluorescence, fluorescence polarization, and more.

• Integrated Microscopy: Automated microscopy with fluorescence, phase contrast, brightfield (HC and color), and brightfield modes.

• Plate Compatibility: 6 to 1,536 well plates.

• Environmental Controls: Equipped with temperature control and carbon dioxide/oxygen control for live-cell assays.

• Modularity: Highly modular and upgradable design to adapt to evolving research needs.

Pros:

• Unparalleled versatility with combined plate reading and imaging.

• High-throughput capabilities for large-scale studies.

• Environmental controls enable advanced cell-based assays.

• Future-proof modular design.

Who it’s ideal for: Labs performing a wide variety of specialized assays, particularly those involving live-cell imaging, complex cellular analysis, or high-throughput screening. It's an investment for labs at the forefront of research requiring the utmost precision and functionality.

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