Emerging Mass Spectrometry Technologies to Improve Intraoperative Diagnostics

Advances in mass spectrometry may reshape the landscape of surgical oncology, improving diagnostic accuracy and potentially impacting post-operative outcomes

Background

Oncological surgery requires highly accurate diagnosis and resection to be sure all traces of the tumor are removed. Frozen section histopathology combined with imaging is the standard method for tumor diagnosis during oncological surgery. Although important resection decisions are made based on the technique, the methods can produce suboptimal diagnostic accuracy of biopsied tissue.

These challenges may soon be upended by a new mass spec technology that leverages molecular feature analysis for improved diagnostic accuracy. Ambient ionization mass spectrometry (AIMS)¹ may soon become a game changer in the operating room, helping to advance the speed and accuracy of tissue biopsy and resection. Such technology has the potential to reshape the surgical oncology landscape by streamlining procedures and positively impacting surgical results.

Challenges of intraoperative diagnostics

Cancer is personal and each case of the disease emerges with unique characteristics and challenges. Presentation, pathology, prognosis, and ultimately treatment strategies all take place amidst the backdrop of the patient’s personal health and physiology. Although each disease scenario is different, universal tools are often used to identify and inform treatment. One such technique is central to identification and subsequent removal of tumor tissue during surgery. Frozen section histopathology coupled with imaging is the current standard for confirming cancerous tissue and informing resection in the operating room. Decades of use and refinement have made the method reliable, but there are important limitations that leave room for improvement.

Frozen section histopathology involves removal of tissue, cryosectioning, and visual detection of morphological features indicative of the tumor. Confirmation of malignancy, typically by an on-site pathologist, means surgeons must then probe deeper and remove additional tissue for subsequent biopsy. The process must be repeated until benign tissue is eventually confirmed thereby delineating the tumor margin. Tumor resection and boundary definition must be performed in all dimensions to ensure clean margins and removal of all signs of cancerous tissue. The process can be tedious and time-consuming (20-30 min per biopsy), requiring numerous tissue biopsies (10-100 or more) throughout the surgery. Post-surgical confirmation must be performed to stratify the initial diagnosis and to ensure no malignant tissue is left behind. If it is, follow up surgery and adjuvant therapy may be needed, placing significant addition burden on the patient and risking residual disease.

Molecular features analysis to assist diagnosis

A very active area of research has focused on molecular feature analysis in assisting tumor diagnosis. Molecular analysis of morphological features and genetic markers is often more accurate in delineating tumorous tissue compared with standard histopathology and imaging techniques alone. Such analysis can provide detailed insight into the tumor type and can lead to a tailored approach towards treatment. Leveraging molecular analysis in the operating room is a challenge, however, one that only recently has begun to be solved.

Novel technologies have arisen to address the medical need for more accurate and sensitive diagnostic tools. One such technology – ambient ionization mass spectrometry (AIMS) – leverages simplified sample preparation and ion analysis capabilities that are well-suited for intraoperative applications. The technology offers the potential to discern benign from cancerous tissue based on distinct mass spectral profiles. In practice, AIMS may prove to be a vastly improved diagnostic for differentiating cancerous and normal tissue at tumor margins during surgery.

Ambient ionization mass spectrometry

Proteins, lipids, and metabolic products of cells can reveal details of cell function or disfunction. In the case of oncogenesis, the increased energetic needs and hypoxic conditions implicit in cancerous tissues create changes that are measurable by sensitive techniques such as mass spectrometry. Using conventional approaches, complex samples rich with molecular information must undergo extensive fractionation using liquid or gas chromatography upstream of MS to derive definitive results. These sample challenges along with the need for complex instrumentation has limited the application of MS in clinical medicine and has been a barrier for the use of MS in intraoperative diagnostics.

AIMS is a technology that avoids the need for extensive sample prep. The approach allows the sample to be ionized directly from liquid or sold sample substrate and guided into the inlet of the MS instrument. Several ambient ionization methods and hardware setups have been described including DESI, DART, LAESI, APCI, APA, and others⁴.

Beyond removing the barrier of complicated and time-consuming sample fractionation, the AIMS ionization process avoids the requirement for low pressure vacuum. Additionally, the analysis can take place under atmospheric conditions, hence the name “ambient”. These attributes negate the requirements for hardware, vacuum pumps, chromatography devices, and other front-end components typically required for MS set ups. The AIMS approach reduces the overall complexity such that multiple sample interfaces, such as surgical tools and operators with varying experience, can be paired with the instrument.

As proof in concept, DESI-MS methods for rapid molecular characterization of resected tissue have been developed to test the validity of the approach for intraoperative tumor diagnostics². Modifications of the technique, including liquid micro-junction surface sample probe (LN-SSP) MS, have subsequently been used in clinical trial analyses of breast cancer assessing the diagnostic capabilities for intraoperative procedures³.

Robotic surgery coupled with MS tissue analysis

The coupling of ambient ionization technologies with robotic surgical applications has the potential for real-time intra-operative diagnostics and surgical decision making. Along these lines, recent investigations have explored modifications of the approach termed rapid evaporative ionization MS (REIMS) used in concert with robotic surgical resection of head and neck cancers⁶.

Robotic surgical platforms including the iKnife⁶, the SpiderMass⁷, the MassSpec Pen⁸, and the Picosecond InfraRed Laser (PIRL)⁹ have thus far demonstrated compatibility with rapid MS analysis of in-situ samples. These surgical energy devices generate aerosolized tissue which can be captured and transferred into the inlet of DESI-MS or other AIMS instrument for analysis. The total time scale from capture to analysis is on the order or tens of seconds, providing near real-time feedback to guide surgical techniques without interrupting workflows.

A recent study titled “Human robotic surgery with intraoperative tissue identification using rapid evaporation ionization mass spectrometry” demonstrated the first human-based usage of the MS tissue identification system (REIMS) with an iKnife robotic surgery device⁵.  In an initial set of experiments involving laser-assisted transoral robotic surgery (TORS), REIMS spectra were recorded over the mass to charge (m/z) range of 100-1500. Notable differences between tissue types were shown in the m/z 560-1000 area where lipid metabolites are easily identifiable. Spectra of the highest relative abundance ions in this mass range were associated with lipid species including glycerophospholipids, ceramides, and glycolipids.

In a second set of experiments focused on minimal access surgery, transaxillary parathyroidectomy was performed to access the parathyroid gland and the iKnife was used to direct surgical aerosol to the REIMS instrument. By sampling at six different tissues sites, collecting a total of 55 spectra, statistically distinct metabolic signatures were identified. The REIMS-enabled surgical system was successful in identifying all tissue types with 100% diagnostic accuracy when comparing MS data with histological results⁵. These results demonstrate that near real-time molecular feature analysis coupled with a robotic system can help navigate through complex anatomical layers with high-resolution during surgery.